IBA Publikation
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DATUM, UNTERSCHRIFT / DATE, SIGNATURE
BANK AUSTRIA CREDITANSTALT, WIEN (IBAN AT04 1100 0006 2280 0100, BIC BKAUATWW), DEUTSCHE BANK MÜNCHEN (IBAN DE16 7007 0024 0238 8270 00, BIC DEUTDEDBMUC)
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IBA Publikation IBA Publikation
L. Micutkova,
M. Hermann,
M. Offerdinger,
M.W. Hess,
A. Matscheski,
H. Pircher,
C. Mück,
H.L. Ebner,
A. Laich,
E. Ferrando-May,
W.. Zwerschke,
L.A. Huber,
P. Jansen-Dürr
S. 1 - 49 Extern doi:10.1002/ijc.26149
Abstract: Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) is an important regulator of cell proliferation and survival, which plays an important role in a variety of epithelial cancers, including prostate cancer, cervical cancer and breast cancer. IGFBP-3 was described as a tumor suppressor in the prostate and identified as a functional cellular target for the E7 oncoprotein of human papillomaviruses. IGFBP-3 interacts with IGF-I outside the cell; however, IGF-independent actions of IGFBP-3 were also described which are mediated by intracellular IGFBP-3, including nuclear IGFBP-3. The mechanisms by which extracellular proteins can reach the nucleus are still largely unknown. We show here that the addition of IGFBP-3 to living cells results in the rapid appearance of nuclear IGFBP-3 by confocal microscopy of IGFBP-3 uptake in live cells, supported by electron microscopy and cell fractionation studies. IGFBP-3 is internalized through a dynamin-dependent pathway, traffics through endocytic compartments and is finally delivered into the nucleus. We observed docking of IGFBP-3 containing structures to the nuclear envelope and found IGFBP-3 containing dot-like structures to permeate the nuclear envelope. In summary, our findings establish the pathway by which this tumor suppressor protein is delivered from extracellular space to the nucleus. Keywords: IGFBP-3 Endocytosis Trafficking Nuclear_Transport Importin Published Online: 2011/11/23 15:16:22 Document Date: 2010/06/01 14:16:00 Object Identifier: 0xc1aa5576 0x0029dc76 Rights: .
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epub.oeaw – Institutionelles Repositorium der Österreichischen Akademie der Wissenschaften epub.oeaw – Institutional Repository of the Austrian Academy of Sciences
A-1011 Wien, Dr. Ignaz Seipel-Platz 2
Tel. +43-1-515 81/DW 3420, Fax +43-1-515 81/DW 3400 http://epub.oeaw.ac.at, e-mail: epub@oeaw.ac.at |