IBA Publikation
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DATUM, UNTERSCHRIFT / DATE, SIGNATURE
BANK AUSTRIA CREDITANSTALT, WIEN (IBAN AT04 1100 0006 2280 0100, BIC BKAUATWW), DEUTSCHE BANK MÜNCHEN (IBAN DE16 7007 0024 0238 8270 00, BIC DEUTDEDBMUC)
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IBA Publikation IBA Publikation
Maria C. Mitterberger,
Institute for Biomedical Aging Research ,
Stefan Lechner,
Institute for Biomedical Aging Research ,
Monika Mattesich,
Innsbruck Medical University,
Andreas Kaiser,
Institute for Biomedical Aging Research ,
Daniela Probst,
Institute for Biomedical Aging Research ,
Nikolaus Wenger,
Institute for Biomedical Aging Research ,
Gerhard Pierer,
Innsbruck Medical University,
Werner Zwerschke,
Institute for Biomedical Aging Research
S. 35 - 48 Elsevier doi:10.1016/j.scr.2012.04.001
Abstract: The main physiological function of adipose-derived stromal/progenitor cells (ASC) is to differentiate into adipocytes. ASC are most likely localized at perivascular sites in adipose tissues and retain the capacity to differentiate into multiple cell types. Although cell surface markers for ASC have been described, there is no complete consensus on the antigen expression pattern that will precisely define these cells. DLK1(PREF1) is an established marker for mouse adipocyte progenitors which inhibits adipogenesis. This suggests that DLK1(PREF1) could be a useful marker to characterize human ASC. The DLK1(PREF1) status of human ASC is however unknown. In the present study we isolated ASC from the heterogeneous stromal vascular fraction of subcutaneous abdominal fat pats of adult women. These cells were selected by their plastic adherence and expanded to passage 5. The ASC were characterized as relatively homogenous cell population with the capacity to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts and the immunophenotype CD105+/CD90+/CD34+/CD31-/FABP4-. The ASC were positive for DLK1(PREF1) which was well expressed in proliferating and density arrested cells but downregulated in the course of adipogenic differentiation. To investigate whether DLK1(PREF1) plays a role in the regulation of adipogenesis in these cells RNAi-mediated knockdown experiments were conducted. Knockdown of DLK1(PREF1) in differentiating ASC resulted in a significant increase of the expression of the adipogenic key regulator PPARγ2 and of the terminal adipogenic differentiation marker FABP4. We conclude that DLK1(PREF1) is well expressed in human ASC and acts as a negative regulator of adipogenesis. Moreover, DLK1(PREF1) could be a useful marker contributing to the characterization of human ASC. Keywords: Human-adipose-derived-stromal-cells Adipogenesis ASC CD31 CD34 CD90 CD105 DLK1(PREF1) FABP4 PPARγ2 Published Online: 2012/07/17 11:05:31 Object Identifier: 0xc1aa5576 0x002b6eba Rights: .
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epub.oeaw – Institutionelles Repositorium der Österreichischen Akademie der Wissenschaften epub.oeaw – Institutional Repository of the Austrian Academy of Sciences
A-1011 Wien, Dr. Ignaz Seipel-Platz 2
Tel. +43-1-515 81/DW 3420, Fax +43-1-515 81/DW 3400 http://epub.oeaw.ac.at, e-mail: epub@oeaw.ac.at |