IBA Publikation
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DATUM, UNTERSCHRIFT / DATE, SIGNATURE
BANK AUSTRIA CREDITANSTALT, WIEN (IBAN AT04 1100 0006 2280 0100, BIC BKAUATWW), DEUTSCHE BANK MÜNCHEN (IBAN DE16 7007 0024 0238 8270 00, BIC DEUTDEDBMUC)
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IBA Publikation IBA Publikation
Aging 2009 1/9
G. Heeren,
M. Rinnerthaler,
P. Laun,
P. von Seyerl,
S. Kössler,
H. Klinger,
M. Hager,
E. Bogengruber,
S. Jarolim,
B. Simon-Nobbe,
C. Schüller,
D. Carmona-Gutierrez,
L. Breitenbach-Koller,
C. Mück,
P. Jansen-Dürr,
A. Criollo,
G. Kroemer,
F. Madeo,
M. Breitenbach
S. 622 - 636 Impact Journals
Abstract: Abstract: Yeast mother cell‐specific aging constitutes a model of replicative aging as it occurs in stem cell populations ofhigher eukaryotes. Here, we present a new long‐lived yeast deletion mutation, afo1 (for aging factor one), that confers a60% increase in replicative lifespan. AFO1/MRPL25 codes for a protein that is contained in the large subunit of themitochondrial ribosome. Double mutant experiments indicate that the longevity‐increasing action of the afo1 mutation isindependent of mitochondrial translation, yet involves the cytoplasmic Tor1p as well as the growth‐controllingtranscription factor Sfp1p. In their final cell cycle, the long‐lived mutant cells do show the phenotypes of yeast apoptosisindicating that the longevity of the mutant is not caused by an inaility to undergo programmed cell death. Furthermore,the afo1 mutation displays high resistance against oxidants. Despite the respiratory deficiency the mutant has paradoxicalincrease in growth rate compared to generic petite mutants. A comparison of the single and double mutant strains for afo1and fob1 shows that the longevity phenotype of afo1 is independen of the formation of ERCs (ribosomal DNA minicircles).AFO1/MRPL25 function establishes a new connection between mitochondria, metabolism and aging. Published Online: 2009/10/29 14:41:29 Object Identifier: 0xc1aa5576 0x0022a1fb Rights: .
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epub.oeaw – Institutionelles Repositorium der Österreichischen Akademie der Wissenschaften epub.oeaw – Institutional Repository of the Austrian Academy of Sciences
A-1011 Wien, Dr. Ignaz Seipel-Platz 2
Tel. +43-1-515 81/DW 3420, Fax +43-1-515 81/DW 3400 http://epub.oeaw.ac.at, e-mail: epub@oeaw.ac.at |