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IBA Publikation


Verlag der Österreichischen Akademie der Wissenschaften
Austrian Academy of Sciences Press
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IBA Publikation
IBA Publikation







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Verlag der Österreichischen Akademie der Wissenschaften
Austrian Academy of Sciences Press
A-1011 Wien, Dr. Ignaz Seipel-Platz 2,
Tel. +43-1-515 81/DW 3420, Fax +43-1-515 81/DW 3400
https://verlag.oeaw.ac.at, e-mail: bestellung.verlag@oeaw.ac.at
UID-Nr.: ATU 16251605, FN 71839x Handelsgericht Wien, DVR: 0096385

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IBA Publikation

Subcellular localization of the human papillomavirus 16 E7 oncoprotein in CaSki cells and its detection in cervical adenocarcinoma and adenocarcinoma in situ

    Kerstin Dreier, René Scheiden, Barbara Lener, Daniela Ehehalt, Haymo Pircher, Elisabeth Müller-Holzner, Ursula Rostek, Andreas Kaiser, Marc Fiedler, Sigrun Ressler, Stefan Lechner, Andreas Widschwendter, Jos Even, Catherine Capesius, Pidder Jansen-Dürr, Werner Zwerschke

IBA Publikation, pp. 54-68, 2010/09/22

doi: 10.1016/j.virol.2010.09.024


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doi:10.1016/j.virol.2010.09.024

Abstract

E7 is the major oncoprotein of high-risk human papillomaviruses (HPV) which causes cervical cancer. To date E7 oncoproteins have not been investigated in cervical adenocarcinoma. In this study we generated a rabbit monoclonal anti-HPV-16 E7 antibody, RabMab42-3, which recognizes a conformational epitope in the E7 carboxy-terminal zinc-finger resulting in a strong increase in the sensitivity for the detection of cell-associated HPV-16 E7 protein relative to conventional polyclonal anti-HPV-16 E7 antibodies. Using RabMab42-3, we show that the subcellular localization of endogenous HPV-16 E7 oncoprotein varies during the cell cycle in cervical cancer cells. Moreover, we demonstrate for the first time that the HPV-16 E7 oncoprotein is abundantly expressed in cervical adenocarcinoma in situ and adenocarcinoma, suggesting an important role of HPV-16 E7 for the development of these tumors. Our findings suggest that the HPV-16 E7 oncoprotein could be a useful marker for the detection of cervical adenocarcinoma and their precursors.